Non-coding RNAs (ncRNAs) are known regulators of gene expression, therefore studying their expression in aging is one step towards completing our understanding of organismal and cell aging. We generated an aging expression atlas of small non-coding RNA with bulk sequencing data of all major mouse tissues (16 organs, 10 timepoints; 1 - 27 months). Expression of micro-RNA (miRNA), one class of ncRNA takes dynamic shapes over the lifespan depending on organ identity, as does gene expression. In addition to these tissue specific molecular aging trajectories, we identified eight deregulated miRNAs during aging in several tissues. The expression of three miRNAs decreased with age (miR-300-3p, miR-487b-3p and miR-541-5p), opposite to five other miRNAs which increased with age (miR-29a-3p, miR-29c-3p, miR-155-5p, miR-184-3p and miR-1895). Predicted targets of these miRNAs are included in extracellular matrix pathways and pathways related to immune system processes. Especially, miR-29c-3p expression increased in all tissues and was also prominently observed circulating in blood. During heterochronic parabiosis, miR-29c-3p expression can be restored to its youthful level in liver. As these results were derived from bulk sequencing data, we collected samples from 15 different brain regions to enhance precision for this complex organ.

Authors:
Viktoria Wagner, Saarland University, Germany
Annika Engel, Saarland University, Germany
Andreas Keller, Saarland University, Germany
Oliver Hahn, Stanford University, United States
Tony Wyss-Coray, Stanford University, United States

About the Presenter(s)
Ms Viktoria Wagner is a University Doctoral Student at Saarland University in Germany

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