Secreted phosphoprotein 1 (SPP1), also known as osteopontin (OPN), is a multifunctional glycoprotein implicated in various diseases. Despite its association with regulatory proteins and pro-inflammatory immune chemokines, the genetic connections between SPP1 and ovarian aging remain largely unexplored. This study aims to fill this knowledge gap by investigating the relationships between SPP1 and ovarian aging through multi-omics data analysis. To comprehend the role of SPP1, spatial transcriptomic analyses were conducted on ovaries from young and aged female mice. The study also included a sample from a 73-year-old individual. In-depth single-cell RNA sequencing analysis was performed to identify associations between SPP1 and key genes such as ITGAV, ITGB1, CD44, MMP3, and FN1. Co-expression analysis focused on revealing correlations, particularly between SPP1 and ITGB1. The findings indicate that SPP1 is a potential gene related to ovarian aging. Spatial transcriptomic analyses revealed a significant decline in SPP1 expression in aged ovaries compared to young ovaries. A similarly low level of SPP1 was observed in the 73-year-old sample. Single-cell RNA sequencing analysis unveiled associations between SPP1 and crucial genes involved in cellular functions. Notably, strong co-expression correlation was identified between SPP1 and ITGB1.

Authors:
Kuan-Hao Tsui, Kaohsiung Veterans General Hospital, Taiwan
Chia-Jung Li, Kaohsiung Veterans General Hospital, Taiwan

About the Presenter(s)
Professor Kuan-Hao Tsui is a University Professor/Principal Lecturer at Kaohsiung Veterans General Hospital in Taiwan

See this presentation on the full schedule – Tuesday Schedule

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